Alkaline Phosphatase Antibody Biotin Conjugated, Rabbit, Polyclonal

Product Overview

• Article Name: Alkaline Phosphatase Antibody Biotin Conjugated, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-200-4635S
• Supplier Catalog Number: 200-4635S
• Alternative Catalog Number: ROC-200-4635S
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Bovine
• Immunogen: Alkaline Phosphatase [Calf Intestine]
• Conjugation: Biotin
• Alternative Names: rabbit anti-Alkaline Phosphatase Antibody biotin Conjugation, biotin conjugated rabbit anti-Alkaline Phosphatase Antibody, Alkaline phosphomonoesterase antibody, ALPI antibody, Glycerophosphatase antibody, IAP antibody, Intestinal alkaline phosphatase antibody, Kasahara isozyme antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• NCBI: 280993
• UniProt: P19111
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Bovine
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:1,000 - 1:10,000, WB: 1:500 - 1:2,000
• Application Notes: Anti-Alkaline Phosphatase Biotin Conjugated Antibody has been tested by ELISA and Western blot. This product is assayed against 1.0 ug of Alkaline Phosphatase in a standard capture ELISA using Peroxidase Conjugated Streptavidin S000-03 and ABTS (2,2-az

Images

Western Blot of Rabbit anti-Alkaline Phosphatase antibody Biotin Conjugated. Lane 1: Alkaline Phosphatase (calf intestine). Load: 50 ng per lane. Primary antibody: Alkaline Phosphatase antibody Biotin conjugated at 1:2,000 for 60 min at RT. Secondary antibody: Peroxidase Streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting (MB-070) for 30 min at RT. Predicted/Observed size: 57 kDa, 57 kDa for Alkaline Phosphatase.