Acid Phosphatase Antibody Biotin Conjugated, Rabbit, Polyclonal

Product Overview

• Article Name: Acid Phosphatase Antibody Biotin Conjugated, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-200-4640S
• Supplier Catalog Number: 200-4640S
• Alternative Catalog Number: ROC-200-4640S
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: DOT
• Species Reactivity: Plant
• Immunogen: Acid Phosphatase [Potato]
• Conjugation: Biotin
• Alternative Names: rabbit anti-Acid Phosphatase Antibody biotin Conjugation, biotin conjugated rabbit anti-Acid Phosphatase Antibody, Acid phosphatase 1 soluble antibody, PAP3, Acid phosphatase of erythrocyte antibody, Adipocyte acid phosphatase antibody, Cytoplasmic phosphotyrosyl protein phosphatase antibody, HAAP antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• NCBI: 37528
• UniProt: Q6J5M8
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Potato
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB: User Optimized
• Application Notes: Anti-Acid Phosphatase Biotin Conjugated Antibody has been tested by dot blot and is suitable to be assayed against 1.0 ug of Acid Phosphatase in a standard capture ELISA using Peroxidase Conjugated Streptavidin S000-03 and ABTS (2,2-azino-bis-[3-ethylb

Images

Dot Blot results of Rabbit Anti-Acid Phosphatase Biotin Conjugated. Antigen: Acid Phosphatase. Blot loaded at 3 fold dilution: 1. 100ng, 2. 33.3ng, 3. 11.1ng, 4. 3.70ng, 5. 1.23ng. Blocking: MB-070 Buffer for 30 minutes at RT. Primary Antibody: Rabbit Anti-Acid Phosphatase Biotin 10µg/mL for 1hr at RT. Secondary Antibody: Streptavidin-HRP (p/n S000-03) at 1:40,000 for 30min at RT. Imaging System ChemiDoc, Filter used: Chemi.