Anti-Alcohol Dehydrogenase Biotin Conjugated Antibody has been tested in ELISA and Western blot. This product is assayed against 1.0 ug of Alcohol Dehydrogenase in a standard capture ELISA using Peroxidase Conjugated Streptavidin S000-03 and ABTS (2,2-
Western Blot of Rabbit anti-Alcohol Dehydrogenase (Yeast) Antibody Biotin Conjugated. Lane 1: Alcohol Dehydrogenase (yeast). Load: 50 ng per lane. Primary antibody: Rabbit anti-Alcohol Dehydrogenase (Yeast) Antibody Biotin Conjugated at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase Streptavidin Antibody (p/n S000-03) at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 37 kDa, 45 kDa for Alcohol Dehydrogenase (yeast).
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