0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Bovine
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:2,000 - 1:10,000, WB: 1:200 - 1:1,000
Application Notes:
Anti-Carbonic Anhydrase II Biotin Conjugated Antibody has been tested against 1.0 ug of Carbonic Anhydrase II in a standard capture ELISA using Peroxidase Conjugated Streptavidin S000-03 and ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) c
ELISA results of purified Rabbit Anti-CARBONIC ANHYDRASE II Biotin Conjugated Antibody tested against Carbonic Anhydrase II. Each well was coated in duplicate with 1.0 µg of Carbonic Anhydrase II. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer MB-060-1000, Streptavidin HRP conjugate, and TMB-1000 substrate.
* VAT and and shipping costs not included. Errors and price changes excepted
