Trypsin Inhibitor Antibody Biotin Conjugated, Rabbit, Polyclonal

Product Overview

• Article Name: Trypsin Inhibitor Antibody Biotin Conjugated, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-200-4679S
• Supplier Catalog Number: 200-4679S
• Alternative Catalog Number: ROC-200-4679S
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Plant
• Immunogen: Anti-Trypsin Inhibitor was produced by repeated immunizations with soy bean trypsin inhibitor.
• Conjugation: Biotin
• Alternative Names: rabbit anti-trypsin inhibitor antibody biotin conjugation, biotin conjugated rabbit anti-trypsin inhibitor antibody, 25 kDa trypsin inhibitor (Protease inhibitor 15 preproprotein) antibody, CRISP8 antibody, KTi antibody, Kti S antibody, KTi3 antibody, KtiS antibody, Kunitz trypsin inhibitor antibody, Kunitz type trypsin inhibitor A antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• NCBI: 547831
• UniProt: P01070
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Soy Bean
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:1,000 - 1:5,000, WB: 1:500 - 1:2,500
• Application Notes: Anti-Trypsin Inhibitor Biotin Conjugated antibody has been tested by ELISA and western blotting. Researchers should determine optimal titers for applications that are not stated below.

Images

Western Blot of Rabbit anti-Soybean Trypsin Inhibitor antibody. Lane 1: ~1 ug of purified Soybean Trypsin Inhibitor Reduced. Lane 2: 0.25 ug of purified Soybean Trypsin Inhibitor Reduced. Lane 3: ~1 ug of purified Soybean Trypsin Inhibitor Non-reduced. Lane 4: 0.25 ug of purified Soybean Trypsin Inhibitor Non-reduced. Primary antibody: Soybean Trypsin Inhibitor antibody at 1:5000 for overnight at 4C. Secondary antibody: Dylight 488 conjugated Streptavidin , 1:5K and Atto 425 conjugated goat anti rabbit, 1:10K at 1.5 hr at RT. Block: MB-070 overnight at 4C. Predicted/Observed size: 24 kDa, 17 kDa for Trypsin. Other band(s): none.

Western Blot of Rabbit anti-Soybean Trypsin Inhibitor antibody. Lane 1: Soybean Trypsin Inhibitor Reduced. Lane 2: Soybean Trypsin Inhibitor Non-Reduced. Lane 3: Streptavidin Reduced. Lane 4: Streptavidin Non-reduced. Load: ~1 ug per lane. Primary antibody: 1:1000 dilution of primary antibody (ON 4 C). Secondary antibody: Dylight 649 conjugated Donkey anti rabbit (611-743-127 lot 20831 1:10K 1.5 hr RT. Block: MB-070 overnight at 4C. Predicted/Observed size: 24 kDa/20kDa for Trypsin, 18.8 kDa/15 kDa for Streptavidin. Other band(s): none.