0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Bovine
Antibody Type:
Primary Antibody
Application Dilute:
IP: 1:100, WB: 1:250
Application Notes:
Anti-Xanthine Oxidase Biotin conjugated antibody has been tested by dot blot and is suitable to be assayed against 1.0 ug of Xanthine Oxidase in a standard capture ELISA using Peroxidase Conjugated Streptavidin S000-03 and ABTS (2,2-azino-bis-[3-ethylb
Dot Blot results of Rabbit Anti-Xanthine Oxidase Antibody Biotin Conjugated. Dots are Xanthine Oxidase at (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng. Blocking: MB-070 for 30 min at RT. Primary Antibody: Rabbit Anti-Xanthine Oxidase Antibody Biotin at 1µg/mL for 1hr at RT. Secondary Antibody: Streptavidin-HRP (p/n S000-03) at 1:40,000 for 30min at RT. Imaged with BioRad ChemiDoc, Chemi filter.
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