0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Lyophilized
Target:
Lima Bean
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:500 - 1:2,000, WB: 1:250
Application Notes:
Anti-Trypsin Inhibitor antibody has been tested by dot blot and assayed against 1.0 ug of Trypsin Inhibitor in a standard capture ELISA using Peroxidase Conjugated Streptavidin S000-03 and ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) cod
Dot Blot results of Rabbit Anti-Trypsin Inhibitor Antibody Biotin Conjugated. Dots are Trypsin Inhibitor at (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng. Blocking: MB-070 for 30 min at RT. Primary Antibody: Rabbit Anti-Trypsin Inhibitor Antibody Biotin at 1µg/mL for 1hr at RT. Secondary Antibody: Streptavidin-HRP (p/n S000-03) at 1:40,000 for 30min at RT. Imaged with BioRad ChemiDoc, Chemi filter.
* VAT and and shipping costs not included. Errors and price changes excepted