0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Lyophilized
Target:
Bovine
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:150,000, IP: 1:100, WB: 1:500 - 1:2,500
Application Notes:
Anti-Ribonuclease A Biotin Conjugated antibody has been tested by ELISA and western blot. This product is assayed against 1.0 ug of Ribonuclease A in a standard capture ELISA using Peroxidase Conjugated Streptavidin S000-03 and ABTS (2,2-azino-bis-[3-e
Western Blot of Rabbit anti-Ribonuclease A Antibody Biotin Conjugated. Lane 1: Ribonuclease A (Bovine Pancreas). Load: 50 ng per lane. Primary antibody: Rabbit anti-Ribonuclease A Antibody Biotin Conjugated at 1:1,000 overnight at 4C. Secondary antibody: HRP streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 16.5 kDa, 16.5 kDa for Ribonuclease A. Other band(s): Possible dimerization.
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