Anti-Avidin Antibody Biotin Conjugated has been tested by ELISA and western blot and is suitable for immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Western Blot of Rabbit anti-Avidin Biotin Conjugated Antibody. Lane 1: Avidin. Lane 2: None. Load: 100 ng per lane. Primary antibody: Avidin antibody at 1:1000 for 1H at RT. Secondary antibody: Peroxidase streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: ~17 kDa, ~17 kDa for monomeric Avidin. Other band(s): None.
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