HSF1 Antibody, IgG1, Clone: [10H8], Rat, Monoclonal

Product Overview

• Article Name: HSF1 Antibody, IgG1, Clone: [10H8], Rat, Monoclonal
• Biozol Catalog Number: ROC-200-501-F50
• Supplier Catalog Number: 200-501-F50
• Alternative Catalog Number: ROC-200-501-F50
• Manufacturer: Rockland Immunochemicals
• Host: Rat
• Category: Antikörper
• Application: FC, IHC, IP, WB
• Species Reactivity: Bovine, Guinea pig, Hamster, Human, Monkey, Mouse, Rabbit, Rat
• Immunogen: HSF1 Antibody was produced in rat by repeated immunizations with purified recombinant HSF1 protein.
• Conjugation: Unconjugated
• Alternative Names: HSTF1, Heat shock factor protein 1, Heat shock transcription factor 1, HSF 1

Product Properties

• Clonality: Monoclonal
• Concentration: 1mg/mL by UV absorbance at 280 nm
• Clone Designation: [10H8]
• Isotype: IgG1
• NCBI: 3297
• UniProt: Q00613
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Human
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:200, Gel Shift: User Optimized, IF Microscopy: 10ug/ml, IP: User Optimized, WB: 1 µg/mL
• Application Notes: Anti-HSF1 Antibody is tested for WB, IP, IF microscopy, and IHC. Expect a band approximately ~85kDa protein in unstressed cell lysates, and an ~95kDa protein in heat shocked cell lysates, corresponding to the molecular mass of inactive and active forms o

Images

Immunofluorescence of rat anti-HSF1 antibody. Tissue: mitotic HeLa cells. Antigen retrieval: Heat Shock. Primary Antibody: HSF1 antibody at 10ug/ml for 1h at RT. Secondary antibody: Fluorescein rat secondary at 1:10,000 for 45 min at RT. Localization: nuclear. Staining: HSF1 granules present as (green) fluorescent signal.