CD8a Fluorescein Antibody, IgG2a, Clone: [53-6.7], FITC, Rat, Monoclonal

Product Overview

• Article Name: CD8a Fluorescein Antibody, IgG2a, Clone: [53-6.7], FITC, Rat, Monoclonal
• Biozol Catalog Number: ROC-200-502-N77
• Supplier Catalog Number: 200-502-N77
• Alternative Catalog Number: ROC-200-502-N77
• Manufacturer: Rockland Immunochemicals
• Host: Rat
• Category: Antikörper
• Application: FC
• Species Reactivity: Mouse
• Immunogen: Anti-CD8a Antibody (Monoclonal) was produced by repeated immunizations with CD8a antigen.
• Conjugation: FITC
• Alternative Names: T-cell surface glycoprotein CD8 alpha chain, T-cell surface glycoprotein Lyt-2, CD8a, Cd8a, Lyt-2, Lyt2

Product Properties

• Clonality: Monoclonal
• Concentration: 0.5mg/mL by UV absorbance at 280 nm
• Clone Designation: [53-6.7]
• Isotype: IgG2a
• NCBI: 12525
• UniProt: P01731
• Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Mouse
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: Flow Cytometry: 10 µL/10 6 cells (0.1 µg), IHC: User Optimized, IP: User Optimized
• Application Notes: Anti-CD8a is tested for FLOW and useful for Immunohistochemistry and Immunoprecipitation using mouse spleen cells, or an appropriate cell type (where indicated). Researchers should determine optimal titers for applications that are not stated.

Images

Flow Cytometry of anti-CD8a Fluorescein Conjugated Monoclonal Antibody. Cells: C57Bl/6 splenocytes. Stimulation: none. Antibody: (Dotted Line) FITC Rat IgG2a isotype control, (Solid Line) Fluorescein Anti-CD8a antibody using 5 ug.