CD86 Fluorescein Antibody, IgG2a, Clone: [GL-1], FITC, Rat, Monoclonal

Product Overview

• Article Name: CD86 Fluorescein Antibody, IgG2a, Clone: [GL-1], FITC, Rat, Monoclonal
• Biozol Catalog Number: ROC-200-502-N90
• Supplier Catalog Number: 200-502-N90
• Alternative Catalog Number: ROC-200-502-N90
• Manufacturer: Rockland Immunochemicals
• Host: Rat
• Category: Antikörper
• Application: FC
• Species Reactivity: Mouse
• Immunogen: Anti-CD86 Antibody (Monoclonal) was produced by repeated immunizations with CD86 antigen.
• Conjugation: FITC
• Alternative Names: T-lymphocyte activation antigen CD86, Activation B7-2 antigen, Early T-cell costimulatory molecule 1, ETC-1, CD86, Cd86

Product Properties

• Clonality: Monoclonal
• Concentration: 0.5mg/mL by UV absorbance at 280 nm
• Clone Designation: [GL-1]
• Isotype: IgG2a
• NCBI: 12524
• UniProt: P42082
• Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Mouse
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: Flow Cytometry: 10 µL/10 6 cells (0.1 µg), IHC: User Optimized, IF Microscopy: User Optimized
• Application Notes: Anti-CD86 is tested for FLOW and useful for Immunohistochemistry and Immunofluorescence using mouse spleen cells, or an appropriate cell type (where indicated). Researchers should determine optimal titers for applications that are not stated.

Images

Flow Cytometry of anti-CD86 Fluorescein Conjugated Monoclonal Antibody. Cells: C57BL/6 splenocytes. Stimulation: LPS for 3 days (right panel). Antibody: (Dotted Line) FITC Rat IgG2a isotype control, (Solid Line) Fluorescein Anti-CD86 antibody using 0.125µg.