CD45.1 Phycoerythrin Antibody, IgG2a, Clone: [A20], RPE, Mouse, Monoclonal

Product Overview

• Article Name: CD45.1 Phycoerythrin Antibody, IgG2a, Clone: [A20], RPE, Mouse, Monoclonal
• Biozol Catalog Number: ROC-200-508-N85
• Supplier Catalog Number: 200-508-N85
• Alternative Catalog Number: ROC-200-508-N85
• Manufacturer: Rockland Immunochemicals
• Host: Mouse
• Category: Antikörper
• Application: FC
• Species Reactivity: Mouse
• Immunogen: Anti-CD45.1 Antibody (Monoclonal) was produced by repeated immunizations with CD45.1 antigen.
• Conjugation: RPE
• Alternative Names: Receptor-type tyrosine-protein phosphatase C, , ukocyte common antigen, L-CA, Lymphocyte antigen 5, Ly-5, T200, CD45, Ptprc, Ly-5

Product Properties

• Clonality: Monoclonal
• Concentration: 0.2 mg/mL by UV absorbance at 280 nm
• Clone Designation: [A20]
• Isotype: IgG2a
• NCBI: 06800
• UniProt: P06800
• Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Mouse
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: Flow Cytometry: 10 µL/10 6 cells (0.1 µg), IHC: User Optimized, IP: User Optimized
• Application Notes: Anti-CD45.1 is tested for Flow Cytometry and useful Immunoprecipitation and Immunohistochemistry using mouse spleen cells, or an appropriate cell type (where indicated). Researchers should determine optimal titers for applications that are not stated.

Images

Flow Cytometry of anti-CD45.1 Phycoerythrin Conjugated Monoclonal Antibody. Cells: C57Bl/6 splenocytes (left panel) or SJL splenocytes (right panel). Stimulation: None. Antibody: 0.5 ug Phycoerythrin Anti-CD45.1 antibody (solid line) or 0.5 ug PE Mouse IgG2a isotype control (dashed line).