Lactoperoxidase Antibody Peroxidase Conjugated, HRP, Sheep, Polyclonal

Product Overview

• Article Name: Lactoperoxidase Antibody Peroxidase Conjugated, HRP, Sheep, Polyclonal
• Biozol Catalog Number: ROC-200-603-146S
• Supplier Catalog Number: 200-603-146S
• Alternative Catalog Number: ROC-200-603-146S
• Manufacturer: Rockland Immunochemicals
• Host: Sheep
• Category: Antikörper
• Application: DOT, WB
• Species Reactivity: Bovine
• Immunogen: Lactoperoxidase [Bovine Milk]
• Conjugation: HRP
• Alternative Names: sheep anti-Lactoperoxidase HRP conjugated Antibody, peroxidase conjugated sheep anti-Lactoperoxidase Antibody, LPO antibody, Salivary peroxidase antibody, SAPX antibody, SPO antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0mg/mL by UV absorbance at 280 nm
• NCBI: 280844
• UniProt: P80025
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Bovine
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:5,000 - 1:20,000, IP: 1:100, WB: 1:500 - 1:5,000
• Application Notes: Anti-Lactoperoxidase Peroxidase Conjugated Antibody has been tested by dot blot and western blot and is suitable to be assayed against 1.0 ug of Lactoperoxidase [Bovine Milk] in a standard capture ELISA using ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-

Images

Rockland Sheep anti Lactoperoxidase antibody (200-601-146 lot 5243) was used to detect Lactoperoxidase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (ON 4 C in MB-070). Detection shown was using Dylight 488 conjugated secondary antibody (605-741-125 lot 21094 1:10K in TBS/MB-070 1 hr RT). Images were collected using the BioRad VersaDoc System