0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Aspergillus niger
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:5,500 - 1:25,000, WB: 1:500 - 1:3,000
Application Notes:
Glucose Oxidase antibody has been tested by dot blot and is suitable to be assayed against 1.0 ug of Glucose Oxidase [Aspergillus niger] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Sheep IgG [H&L] (Rabbit) code 613-4302 and (A
Dot Blot results of Sheep Anti-Glucose Oxidase Antibody. Dots are Glucose Oxidase at (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng. Blocking: MB-070 for 30 min at RT. Primary Antibody: Sheep Anti-Glucose Oxidase Antibody at 10µg/mL for 1hr at RT. Secondary Antibody: Donkey Anti-Sheep-HRP (p/n 613-703-168) at 1:40,000 for 30min at RT. Imaged with BioRad ChemiDoc, Chemi filter.
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