This IgY fraction antibody was prepared from eggs of chickens laid after repeated immunizations with Protein A [Staphylococcus aureus].
Conjugation:
Unconjugated
Alternative Names:
chicken anti-Protein A Antibody, chicken anti Protein A, chicken anti PA
Clonality:
Polyclonal
Concentration:
1 mg/ml by UV absorbance at 280 nm
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:500 - 1:2,000, WB: 1:500 - 1:2,000
Application Notes:
Anti-PROTEIN A (CHICKEN) Antibody has been tested by western blotting and suitable for ELISA. Although not tested, this antibody may also be useful for neutralization assays, immunohistochemistry, flow Cytometry, immunoprecipitation, immunodiffusion, con
Dot Blot of Chicken Anti-Protein A Antibody. Dilutions of Protein A: (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.7ng, and (5) 1.23ng. Top Blot incubated with Primary Antibody: 1µg/mL of Anti-Protein A Antibody for 1 hour at room temp. Bottom Blot incubated with Secondary Only: Rabbit Fab2 anti-Chicken Peroxidase conjugated (p/n 703-403-002) 1:40,000 for 30 min at room temp. Blocking Buffer: BlockOut Universal Blocker (p/n MB-073). Exposure 7 sec.
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