This IgY fraction antibody was prepared from eggs of chickens laid after repeated immunizations with a synthetic peptide corresponding to aa 2-32 of Human MIF conjugated to keyhole limpet hemocyanin (KLH). MIF is a proinflammatory cytokine that plays an important role in systemic inflammatory events.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Lyophilized
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:1,000 - 1:5,000, WB: 1:500 - 1:2,000
Application Notes:
This IgY fraction antibody of anti-Human macrophage migration inhibitory factor (MIF) has been tested for use in ELISA and immunoblotting. Although not tested, this antibody may also be useful for neutralization assays, immunohistochemistry and flow cyto
Western blot analysis of ROCKLAND Immunochemicals IgY fraction of Chicken anti-Human MIF polyclonal antibody. Lane 2 shows the detection of 100 ng of recombinant MIF present with a single band at 12.3 kDa. Similar detection of MIF will occur when human serum is analyzed. In lane 1 no reaction is observed in the control. A 4-20% gradient gel was used to separate the proteins by SDS-PAGE. The protein was transferred to nitro-cellulose using standard methods. After blocking the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:5,000 dilution of IRDye(TM)800 conjugated Gt-a-Chicken Rabbit IgG [H&L] (code 603-132-126) for 1 h at room temperature. LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.
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