Neurofilament L Antibody, Gallus, Polyclonal

Catalog Number: ROC-200-901-D83
Article Name: Neurofilament L Antibody, Gallus, Polyclonal
Biozol Catalog Number: ROC-200-901-D83
Supplier Catalog Number: 200-901-D83
Alternative Catalog Number: ROC-200-901-D83
Manufacturer: Rockland Immunochemicals
Host: Gallus
Category: Antikörper
Application: IF, WB
Species Reactivity: Bovine, Gallus, Human, Mouse, Rat
Immunogen: Anti-Neurofilament L Antibody was produced by repeated immunizations with preparations of bovine spinal cord intermediate filaments.
Conjugation: Unconjugated
Alternative Names: Neurofilament light polypeptide, NF-L, 68 kDa neurofilament protein, Neurofilament triplet L protein
Clonality: Polyclonal
NCBI: 4747
UniProt: P02548
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form: Liquid
Target: Bovine
Antibody Type: Primary Antibody
Application Dilute: IF Microscopy: 1:5000, WB: 1:10000
Application Notes: Anti-Neurofilament L antibody is tested for use in Western Blotting and IF. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 68 kDa in size corresponding to the NF-L proteins in Western blots in the a
Western Blot of Chicken anti-Neurofilament L antibody. Lane 1: Rat cortex lysate. Lane 2: none. Load: 20 µg per lane. Primary antibody: Neurofilament L antibody at 1:10,000 for overnight at 4C. Secondary antibody: IRDye800(TM) chicken secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 68 kDa for Neurofilament L. Other band(s): none.