Vimentin Antibody, Gallus, Polyclonal

Product Overview

• Article Name: Vimentin Antibody, Gallus, Polyclonal
• Biozol Catalog Number: ROC-200-901-E54
• Supplier Catalog Number: 200-901-E54
• Alternative Catalog Number: ROC-200-901-E54
• Manufacturer: Rockland Immunochemicals
• Host: Gallus
• Category: Antikörper
• Application: IF, Multiplex Assay, WB
• Species Reactivity: Bovine, Human, Mouse, Rat
• Immunogen: Anti-Vimentin Monoclonal Antibody was produced in chicken by repeated immunizations with recombinant human vimentin extensively purified from E. coli.
• Conjugation: Unconjugated
• Alternative Names: VIM

Product Properties

• Clonality: Polyclonal
• Concentration: Titrated value sufficient to run approximately 10 mini blots.
• NCBI: 7431
• UniProt: P08670
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid
• Target: Human
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:10,000, IF Microscopy: 1:500, WB: 1:1000
• Application Notes: Anti-Vimentin antibody is tested for use in Western Blotting and IF. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 50 kDa in size corresponding to the vimentin proteins in Western blots in the appr

Images

Immunofluorescence Microscopy of Chicken anti-Vimentin antibody. Tissue: Mixed neuron/glial cultures. Fixation: 0.5% PFA Antigen retrieval: not required. Primary antibody: Vimentin antibody at 1:500 for 1 h at RT. Secondary antibody: Fluorescein chicken secondary antibody at 1:10,000 for 45 min at RT. Localization: Vimentin is found in many kinds of mesenchymal and epithelial cells as well as developing neuronal and astrocytic precursor cells in the CNS. Staining: Vimentin Antibody as green fluorescent signal, GFAP Antibody as red, Vimentin and GFAP as yellow.