Anti-SIRT3 antibody was prepared from chicken egg fractions produced by repeated immunizations with a 17 amino acid synthetic peptide near the internal region of human SIRT3.
Conjugation:
Unconjugated
Alternative Names:
SIRT3 Antibody, SIR2L3, SIR2L3, NAD-dependent protein deacetylase sirtuin-3, mitochondrial, Regulatory protein SIR2 homolog 3, hSIRT3
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:10,000 - 1:20,000, WB: 1-2 µg/mL
Application Notes:
Anti-SIRT3 Antibody has been tested for use in ELISA and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 44 kDa in Western Blots of specific cell lysates and tissues.
Western Blot of SIRT3 antibody. Lane 1: Mouse heart tissue lysate with SIRT3 antibody at 1 µg/mL. Lane 2: Mouse heart tissue lysate with SIRT3 antibody at 2 µg/mL. Secondary antibody: Peroxidase chicken secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 44 kDa, 45 kDa for SIRT3. Other band(s): SIRT3 splice variants and isoforms.
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