Anti-SIRT7 antibody was prepared from chicken egg fractions produced by repeated immunizations with a 19 amino acid synthetic peptide near the N-terminus of human SIRT7.
Conjugation:
Unconjugated
Alternative Names:
SIRT7 Antibody, SIR2L7, SIR2L7, NAD-dependent protein deacetylase sirtuin-7, Regulatory protein SIR2 homolog 7
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:5,000 - 1:10,000, WB: .5-1 µg/mL
Application Notes:
Anti-SIRT7 Antibody has been tested for use in ELISA and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 45 kDa in Western Blots of specific cell lysates and tissues.
Western Blot of SIRT7 antibody. Lane 1: 293 cell lysate with SIRT7 antibody at 0.5 µg/mL. Lane 2: 293 cell lysate with SIRT7 antibody at 1 µg/mL. Secondary antibody: Peroxidase chicken secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 45 kDa, 47 kDa for SIRT7. Other band(s): SIRT7 splice variants and isoforms.
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