Anti-Tubulin antibody was prepared from chicken egg fractions produced by repeated immunizations with a 16 amino acid synthetic peptide near the N-terminus of human Tubulin.
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:5000-1:10000, WB: 0.5 - 1 µg/mL
Application Notes:
Anti-Alpha-tubulin Antibody has been tested for use in ELISA and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 50 kDa in Western Blots of specific cell lysates and tissues.
Western Blot of Tubulin antibody. Lane 1: Rat brain tissue lysate with Tubulin antibody at 0.5 µg/mL. Lane 2: Rat brain tissue lysate with Tubulin antibody at 1 µg/mL. Load: 35 µg per lane. Secondary antibody: Peroxidase anti-chicken secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 50 kDa, 52 kDa for Tubulin. Other band(s): Tubulin splice variants and isoforms.
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