Anti-Beta-actin antibody was prepared from chicken egg fractions produced by repeated immunizations with a 16 amino acid synthetic peptide from near the C-terminus of human beta-actin.
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:10,000-1:20,000, WB: 1 - 2 µg/mL
Application Notes:
Anti-Beta-actin Antibody has been tested for use in ELISA and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 42 kDa in Western Blots of specific cell lysates and tissues.
Western Blot of beta-actin antibody. Lane 1: HeLa cell lysate with beta-actin antibody at 1 µg/mL. Lane 2: HeLa cell lysate with beta-actin antibody at 2 µg/mL. Load: 35 µg per lane. Secondary antibody: Peroxidase chicken secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 41.7 kDa, 46 kDa for beta-actin. Other band(s): beta-actin splice variants and isoforms.
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