This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein raised in yeast, corresponding to the 83 amino acids of the mature bovine CXCL10 protein.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Lyophilized
Target:
Bovine
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:500, WB: 1 µg/mL
Application Notes:
This protein-A purified CXCL10 antibody has been tested by ELISA and Western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 9.3 kDa in size corresponding to bovine CXCL10 protein by western b
Western blot using Rocklands protein-A purified anti-bovine CXCL10 antibody shows detection of recombinant bovine CXCL10 at 9.3 kDa (arrowhead) raised in yeast. The protein was purified and resolved by SDS-PAGE, then transferred to PVDF membrane. Membrane was blocked with 3% BSA (BSA-30, diluted 1:10), and probed with 1 µg/mL primary antibody overnight at 4C. After washing, membrane was probed with IRDye800(TM) Conjugated Goat Anti-Rabbit IgG (p/n 611-132-122) for 45 min at room temperature.
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