Rabbit IgG anti-Chicken IgG (H+L)-HRPO, MinX none

Product Overview

• Article Name: Rabbit IgG anti-Chicken IgG (H+L)-HRPO, MinX none
• Biozol Catalog Number: ROC-203-4302
• Supplier Catalog Number: 203-4302
• Alternative Catalog Number: ROC-203-4302
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Gallus
• Immunogen: Chicken IgG whole molecule
• Conjugation: HRPO
• Alternative Names: rabbit anti-Chicken IgG Antibody Peroxidase Conjugated, rabbit anti-Chicken IgG HRP Conjugated Antibody, rabbit anti-Chicken IgY Antibody Peroxidase Conjugated, rabbit anti-Chicken IgY HRP Conjugated Antibody
• Format: IgG
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 10.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Chicken
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:10,000
• Application Notes: Anti-Chicken IgG Peroxidase Antibody has been tested by ELISA. This product has been assayed against 1.0 ug of Chicken IgG in a standard capture ELISA using ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code ABTS-100 as a substrate for 3

Images

ELISA Results of Rabbit Anti-Chicken IgG/IgY Antibody Peroxidase Conjugated. Each well was coated in duplicate with 1.0 µg of Chicken IgG/IgY (p/n 003-0102). The working dilution is 17,200. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using TMB substrate (p/n TMBE-1000).