Anti-Human IgG F(c) monoclonal antibody was produced by repeated immunization with Human IgG F(c) fragment in mice.
Conjugation:
Unconjugated
Alternative Names:
Human IgG F(c) Antibody, Human IgG Fc Antibody, Ms-a-Human IgG Fc, Human IgG F(c) Antibody in Mouse, Human Secondary Antibody
Format:
IgG1
Target Specificity:
IgG (Fc)
Cross-Adsorption (MinX):
no cross-adsorbtion
Clonality:
Monoclonal
Concentration:
1.0 mg/ml by UV absorbance at 280 nm
Clone Designation:
[3D8.H7.D4]
Isotype:
IgG1
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Secondary Antibody
Application Dilute:
ELISA: 1-:1000-1:10000, WB: 1:1000 - 1:2000
Application Notes:
Human IgG F(c) antibody has been tested by ELISA and Western Blot. This product is suitable for immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection sho
Western Blot of Mouse anti-Human F(c) antibody. Lane 1: Human IgG (p/n 009-0102). Lane 2: Human Fc (p/n 009-0103). Lane 3: Human Fab (p/n 009-0105). Lane 4: Human Fab2 (p/n 009-0104). Lane 5: Human IgM (p/n 009-0107). Lane 6: Human IgM Fc5µ (p/n 009-0131). Load: 50 ng per lane. Primary antibody: Anti-Human F(c) monoclonal antibody at 1:1000 for overnight at 4C. Secondary antibody: HRP mouse secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted MW: ~28kDa. Observed MW: ~33kDa for Human Fc.
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