Mouse IgG anti-Human IgG (H+L)-FITC, MinX none

Product Overview

• Article Name: Mouse IgG anti-Human IgG (H+L)-FITC, MinX none
• Biozol Catalog Number: ROC-209-3202
• Supplier Catalog Number: 209-3202
• Alternative Catalog Number: ROC-209-3202
• Manufacturer: Rockland Immunochemicals
• Host: Mouse
• Category: Antikörper
• Application: FLISA,FACS,IF
• Species Reactivity: Human
• Immunogen: Human IgG whole molecule
• Conjugation: FITC
• Alternative Names: Mouse anti-Human IgG Fluorescein Conjugated Antibody, mouse anti-Human IgG FITC Conjugated Antibody, mouse anti-Human IgG Antibody Fluorescein Conjugation
• Format: IgG
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 10 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Human
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: FLISA: 1:10,000 - 1:50,000, Flow Cytometry: 1:500 - 1:2,500, IF Microscopy: 1:1,000 - 1:5,000
• Application Notes: Anti-Human IgG (H&L) Fluorescein is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing variou

Images

Rockland primary and DyLight conjugated secondary antibodies were used to detect: Human transferrin (1 109-4134, green 2 611-743-127), Alpha 1 anti trypsin (1 100-101-147, red 2 605-742-125), and Human IgG (1 109-3102, Blue 2 610-741-124) in a multiplex fluorescent western blot of human serum. Each primary antibody was diluted to 1:1000 in blocking buffer (MB-070) and incubated for 2 hrs at RT. Secondary antibodies diluted 1:10000 in blocking buffer and incubated ~ 1hr at 4C. Imaged using the Biorad VersaDoc 4000.