The whole rabbit serum used to produce this IgG fraction antibody was prepared by repeated immunizations with recombinant human IL-1a produced in E.coli. The MW of the recombinant IL-1a was 17,000. This is the cleavage site generated by the IL-1b converting enzyme (ICE, capase-1).
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:1,000 - 1:5,000, Flow Cytometry: User Optimized, IHC: 1:100 to 1:200, WB: 1:500 - 1:2,000
Application Notes:
This IgG fraction antibody of anti-Human IL-1a has been tested for use in immunoblotting. This antibody is suitable for use in ELISA, neutralizations, immunohistochemistry, and flow cytometry. It recognizes the 17,000 MW mature IL-1a. Reactivity in other
Western Blot of Rabbit Anti-Human IL-1 alpha Antibody. Lane 1: Opal Prestained Molecular Weight Ladder (p/n MB-210-0500). Lane 2: IL-1 alpha [50ng]. Lane 3: MEF WC lysate [10ng] (p/n W10-001-371). Lane 4: MEF LPS stimulated [10ng]. Blocking: BlockOut Buffer (p/n MB-073) for 30min at RT. Primary Antibody: Anti-IL-1a at 1µg/mL overnight at 4C. Secondary Antibody: Goat anti-Rabbit HRP (p/n 611-103-122) at 1:70, 000 for 30min at RT.
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