The whole rabbit serum used to produce this IgG fraction antibody was prepared by repeated immunizations with recombinant human sIL-1Ra produced in E.coli.
This IgG fraction antibody of anti-Human IL-1 Receptor Antagonist has been tested in western blot and suitable for use in neutralizations, ELISA, radioimmunoassays, immunohistochemistry and flow cytometry. Reactivity in other immunoassays is unknown. Thi
Western Blot of Rabbit Anti-Human IL-1 Receptor Antagonist Antibody. Lane 1: Recombinant Human IL-1 RA Protein [0.05µg] Reduced.Lane 2: Opal Prestained Molecular Weight Marker (p/n MB-210-0500). Lane 3: Recombinant Human IL-1 RA Protein [0.05µg] Non-Reduced. Primary Antibody: Anti-Hu IL-1RA Antibody at 1:800 overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP Antibody (p/n 611-1302) at 1:40,000 for 30 mins at RT. Blocking Buffer: BlockOut Universal Buffer (p/n MB-073) for 1hr at RT. Excepted Molecular Weight: ~18kDa. Exposure: 7.193 secs.
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