GM-CSF Antibody, Rabbit, Polyclonal

Product Overview

• Article Name: GM-CSF Antibody, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-209-401-314
• Supplier Catalog Number: 209-401-314
• Alternative Catalog Number: ROC-209-401-314
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: WB
• Species Reactivity: Human
• Immunogen: This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human GM-CSF protein.
• Conjugation: Unconjugated
• Alternative Names: rabbit anti-GM-CSF Antibody, GM-CSF, rabbit anti-Colony-stimulating factor, CSF, sargramostim and molgramostin

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL
• NCBI: 1437
• UniProt: P04141
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Human
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:1,000 - 1:5,000, WB: 1:500 - 1:2,000
• Application Notes: This purified antibody has been tested for use in western blotting. Reactivity is also expected in neutralizations, ELISA, radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be <10 pg/µl by the LAL method. By western blot a

Images

Western blot using Rocklands anti-Human GM-CSF antibody shows detection of a band ~15 kDa in size corresponding to recombinant human GM-CSF (lane 1). Molecular weight markers are also shown (M). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1,000 dilution. Detection occurred using DyLight(TM)649 conjugated anti-Rabbit IgG (p/n 611-143-122) secondary antibody diluted 1:20,000 in blocking buffer (p/n MB-070). Image was captured using VersaDoc(TM) MP 4000 imaging system (Bio-Rad).