This Protein-A purified antibody was prepared by repeated immunizations with an MBP-tagged recombinant protein produced in E.coli corresponding to mature human NAG-1 protein. Cross reactivity to MBP was removed via cross-adsorption chromatography.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:500, WB: 1:800 - 1:1500
Application Notes:
This Protein-A purified, MBP-cross-adsorbed NaG-1 antibody has been tested by ELISA and Western Blot of human and mouse NAG-1 protein. For detection of NAG-1 in human serum, a sandwich ELISA is suggested using this antibody in combination with anti-NAG-1
Western blot using Rocklands affinity purified anti-Human NAG-1 antibody shows detection of a 42 kDa band corresponding to purified recombinant fusion protein MBP-human NAG-1. Samples were electrophoresed on a 4-20% gradient gel under reducing conditions. Primary antibody was used at 1:1000 in 1% BSA-TBST overnight at 4C. DyLight649(TM) Goat Anti-Rabbit IgG secondary antibody (p/n 611-143-122) was used at 1:20,000 in Blocking Buffer for Fluorescent Western Blotting (MB-070) for 30 min at room temperature. Molecular weight estimation was made by comparison to prestained MW markers. Image was captured using the BioRad Versadoc(TM) 4000MP Imaging System. Other detection systems will yield similar results.
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