MIP-1 beta Antibody, Rabbit, Polyclonal

Product Overview

• Article Name: MIP-1 beta Antibody, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-209-401-B97
• Supplier Catalog Number: 209-401-B97
• Alternative Catalog Number: ROC-209-401-B97
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: WB
• Species Reactivity: Human
• Immunogen: This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human MIP-1ß protein.
• Conjugation: Unconjugated
• Alternative Names: rabbit anti-MIP-1 beta Antibody, rabbit anti-MIP-1b Antibody, CCL4, C-C motif chemokine 4, Small-inducible cytokine A4, Macrophage inflammatory protein 1-beta, MIP-1-beta, ACT-2, T-cell activation protein 2, Protein H400, Lymphocyte activation gene 1 protein, LAG-1, HC21, G-26 T-lymphocyte-secreted protein, MIP-1ß

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• NCBI: 6351
• UniProt: P13236
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Human
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:10,000, WB: 1:1,000
• Application Notes: This purified antibody has been tested in western blotting. Reactivity is also expected in ELISA, neutralizations, radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be <10 pg/µl by the LAL method. By western blot a band app

Images

Rocklands anti-Human MIP-1ß antibody shows detection of a band ~15 kDa in size corresponding to recombinant human MIP-1ß. Molecular weight markers are also shown (left). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1,000 dilution. Detection occurred using peroxidase conjugated anti-Rabbit IgG (p/n 611-103-122) secondary antibody diluted 1:40,000 in blocking buffer (p/n MB-070) for 30 min at RT followed by reaction with FemtoMax(TM) chemiluminescent substrate. Image was captured using VersaDoc(TM) MP 4000 imaging system (Bio-Rad).