VEGF Antibody Peroxidase Conjugated, HRP, Rabbit, Polyclonal

Product Overview

• Article Name: VEGF Antibody Peroxidase Conjugated, HRP, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-209-403-B99
• Supplier Catalog Number: 209-403-B99
• Alternative Catalog Number: ROC-209-403-B99
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: WB
• Species Reactivity: Human
• Immunogen: This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human VEGF-165 protein.
• Conjugation: HRP
• Alternative Names: rabbit anti-VEGF Peroxidase Conjugated antibody, rabbit anti-VEGF-165 Peroxidase Conjugated antibody, Vascular endothelial growth factor A, VEGF-A, VEGF-165, VEGF165, VEGF isoform L, Vascular permeability factor, VPF

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• NCBI: 7422
• UniProt: P15692
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Human
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: IHC: 1:500-1:2,500, WB: 1:1,000-1:5,000
• Application Notes: This protein-A purified antibody has been tested for use in western blotting. Reactivity in other assays has not been determined. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 22 kDa in size correspon

Images

Western Blot showing detection of Recombinant Human VEGF-165. 50ng of protein (Lane 1) was run on a 4-20% gel and transferred to 0.45 µm nitrocellulose. After blocking with 1% BSA-TTBS (p/n MB-013, diluted to 1X) 30 min at 20C, Anti-VEGF-165 (RABBIT) Antibody Peroxidase Conjugate (p/n 209-403-B99) secondary antibody was used at 1:1000 in Blocking Buffer for Fluorescent Western Blotting (p/n MB-070) and imaged using the Bio-Rad VersaDoc 4000 MP. Arrow indicates correct 19 kDa molecular weight position expected for rH-VEGF-165.