This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-33 protein.
Conjugation:
Biotin
Alternative Names:
rabbit anti-Interleukin-33 biotin conjugated antibody, rabbit anti-IL-33 biotin conjugated antibody, Interleukin-1 family member 11, IL-1F11, nuclear factor from high endothelial venules and NF-HEV
This purified antibody has been tested in western blotting and suitable for ELISA. By western blot a band approximately 18 kDa in size corresponding to mature human IL-32alpha protein is expected in the appropriate cell lysate or extract. Specific conditions
Western blot using Rocklands anti-Human IL-33 antibody shows detection of a band ~18 kDa in size corresponding to recombinant human IL-33 (lane 1). The identity of the higher molecular weight band is unknown. Molecular weight markers are also shown (M). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1,000 dilution. Detection occurred using peroxidase conjugated anti-Rabbit IgG (p/n 611-103-122) secondary antibody diluted 1:40,000 in blocking buffer (p/n MB-070) for 30 min at RT followed by reaction with FemtoMax(TM) chemiluminescent substrate. Image was captured using VersaDoc(TM) MP 4000 imaging system (Bio-Rad).
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