VEGF Antibody Biotin Conjugated, Rabbit, Polyclonal

Product Overview

• Article Name: VEGF Antibody Biotin Conjugated, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-209-406-B99
• Supplier Catalog Number: 209-406-B99
• Alternative Catalog Number: ROC-209-406-B99
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: VEGF Antibody Biotin Conjugated was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human VEGF-165 protein.
• Conjugation: Biotin
• Alternative Names: rabbit anti-VEGF Biotin Conjugated antibody, rabbit anti-VEGF-165 Biotin Conjugated antibody, Vascular endothelial growth factor A, VEGF-A, VEGF-165, VEGF165, VEGF isoform L, Vascular permeability factor, VPF

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• NCBI: 7422
• UniProt: P15692
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Human
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:10,000 - 1:50,000, IHC: 1:500-1:2,500, WB: 1:2,000 - 1:10,000
• Application Notes: VEGF conjugated antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 22 kDa in size corresponding to monomeric human VEGF-165 protein by wester

Images

Western Blot of Rabbit anti-VEGF-165 Antibody Biotin Conjugated. Lane 1: VEGF-165 Recombinant Protein. Load: 50 ng per lane. Primary antibody: Rabbit anti-VEGF-165 Antibody Biotin Conjugated at 1:1,000 overnight at 4C. Secondary antibody: HRP Streptavidin (S000-03) at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 22 kDa, 22 kDa for VEGF-165 Isoform.