Anti-TSLP antibody was prepared from rabbit antiserum after repeated immunizations with a truncated form of recombinant human TSLP protein from E. coli.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:20,000 - 1:50,000, WB: 1:1000-1:2000
Application Notes:
TSLP purified antibody has been tested in western blotting and suitable in ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 18 kDa in size corresponding to the human TSLP protein by western blotti
Western blot of Biotin conjugated Rabbit Anti-TSLP primary antibody. Lane 1: Human TSLP. Lane 2: None. Load: 50 ng per lane. Primary antibody: TSLP Biotin conjugated antibody at 1:1,000 for 60 min at RT. Secondary antibody: Peroxidase streptavidin secondary antibody at 1:40,000 for 30 min at RT. Blocking: MB-070 for 30 min at RT. Observed/Predicted size: 13 kDa, 13 kDa for Human TSLP. Other band(s): None.
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