Rabbit IgG anti-Human IgG (H+L)-FITC, MinX none

Product Overview

• Article Name: Rabbit IgG anti-Human IgG (H+L)-FITC, MinX none
• Biozol Catalog Number: ROC-209-4202
• Supplier Catalog Number: 209-4202
• Alternative Catalog Number: ROC-209-4202
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: FLISA,FACS,IF
• Species Reactivity: Human
• Immunogen: Human IgG whole molecule
• Conjugation: FITC
• Alternative Names: rabbit anti-Human IgG Fluorescein Conjugated Antibody, rabbit anti-Human IgG FITC Conjugated Antibody, rabbit anti-Human IgG Antibody Fluorescein Conjugation
• Format: IgG
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 10 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Human
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: FLISA: 1:10,000 - 1:50,000, Flow Cytometry: 1:500 - 1:2,500, IF Microscopy: 1:1,000 - 1:5,000
• Application Notes: Anti-Human IgG (H&L) Fluorescein is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing variou

Images

IgG Secretion. A) ARH-77, B) CCL-119, C) SA13. Both ARH-77 and SA13 cell lines display high fluorescence readouts while CCL-119 shows little fluorescence. Red circles correspond to MBs that contained cells and displayed high levels of immunoprecipitation. Light fluorescent spot in (B) is attributed to a reflection off an out of focus bubble on MB surface. By day 1 the fluorescent images showed that some wells took on a speckled appearance that continued to intensify over time (Fig. 1). By day 4 distinct differences could be discerned between the three cell lines. The fluorescence speckle pattern signifies detection of immunoprecipitation (IP) between secreted IgG and FITC labeled alpha-IgG added to the cell culture media. Figure 1. PMID: 25079889.