Transferrin Antibody Biotin Conjugated, Rabbit, Polyclonal

Product Overview

• Article Name: Transferrin Antibody Biotin Conjugated, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-209-4634-0100
• Supplier Catalog Number: 209-4634-0100
• Alternative Catalog Number: ROC-209-4634-0100
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Transferrin [Human Serum]
• Conjugation: Biotin
• Alternative Names: rabbit anti-Transferrin Antibody Biotin Conjugation, Apotransferrin antibody, Beta 1 metal binding globulin antibody, DKFZp781D0156 antibody, PRO1400 antibody, PRO1557 antibody, PRO2086 antibody, Serotransferrin precursor antibody, Siderophilin antibody, TF antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• NCBI: 7018
• UniProt: P02787
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Human
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:20,000 - 1:150,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Application Notes: Anti-Transferrin Antibody Biotin Conjugated has been tested for use in Immunoblotting and ELISA and is suitable for immunohistochemistry, immunofluorescence microscopy, as well as other antibody based assays. Optimal concentrations should be determined b

Images

Western Blot of Rabbit anti-Transferrin antibody. Lane 1: molecular weight. Lane 2: anti-Transferrin (green), Goat-anti-Alpha-1-Anti-Trypsin, and Mouse-a-GST were used in a multiplex system to detect target proteins under reducing conditions in albumin depleted human serum with 320 ng of added GST. Load: 35 µg per lane. Primary antibody: Transferrin antibody at 1:1000 for overnight at 4C. Secondary antibody: DyLight549 Donkey anti-Rabbit IgG (green), DyLight 488 Donkey anti-Mouse IgG (blue), and DyLight 649 Donkey anti-Goat IgG (red) at 1:10,000 for 45 min at RT. Block: 2.5% Blotto, 2.5% BSA, 0.02% Tween over night at 4C. Other band(s): none.

Western Blot of primary and Dylight conjugated secondary antibodies. Lane 1: molecular weight. Lane 2: Rabbit anti-Human transferrin (green), Alpha 1 anti trypsin (red), and Human IgG (Blue) in a multiplex fluorescent western blot of human serum. Primary antibody: Each primary antibody was diluted to 1:1000 for 2 hrs at RT. Secondary antibody: Rabbit IgG (H&L) Antibody DyLight(TM) 649 Conjugated (green), Goat IgG (H&L) Antibody DyLight(TM) 549 Conjugated (red), Mouse IgG (H&L) Antibody DyLight(TM) 488 Conjugated (blue)secondary antibody at 1:10,000 for 45 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting - MB-070 for 1hr at 4C. Other band(s): none.

Western Blot of Biotin conjugated Rabbit Anti-Transferrin primary antibody. Lane 1: human depleted serum. Lane 2: None. Load: Unknown. Primary antibody: Biotin conjugated Rabbit Anti-Transferrin at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 74 kDa, 74 kDa for Transferrin. Other band(s): None.