0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Mouse
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:750,000, WB: 1:1000
Application Notes:
This protein-A purified antibody has been tested for use western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 75.5 kDa in size corresponding to Esrp-1 by western blotting in the appropriate
Anti-ESRP-1 by western blot shows detection of ESRP-1 seen in lane 2. Lane 1: GFP-transfected-293T cell extracts, Lane 2: ESRP-1-transfected-293T cell extracts, Lane 3: ESRP2-transfected 293T cell lysates. Briefly, each lane contains approximately 5 µg of lysate. Primary antibody was used at a 1:1000 dilution in PBS-T plus milk, and reacted for 1hr at room temperature. The membrane was washed and reacted with a 1:10,000 dilution of an anti-mouse ECL antibody for 1hr at room temperature. Molecular weight estimation was made by comparison to prestained MW markers.
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