Esrp-1/2 Antibody, IgG1, Clone: [23A7.C9], Mouse, Monoclonal

Catalog Number: ROC-210-301-C31
Article Name: Esrp-1/2 Antibody, IgG1, Clone: [23A7.C9], Mouse, Monoclonal
Biozol Catalog Number: ROC-210-301-C31
Supplier Catalog Number: 210-301-C31
Alternative Catalog Number: ROC-210-301-C31
Manufacturer: Rockland Immunochemicals
Host: Mouse
Category: Antikörper
Application: WB
Species Reactivity: Mouse
Immunogen: Anti-Esrp-1/2 was produced by repeated immunizations of full length recombinant mouse Esrp-1 fusion protein.
Conjugation: Unconjugated
Alternative Names: mouse anti-Esrp1/2 antibody, mouse anti-Esrp1 antibody, mouse anti-Ersp2 antibody, Rbm35a, Epithelial splicing regulatory protein 1, RNA-binding protein 35A, RNA-binding motif protein 35A, RNA-binding protein 35B, RNA-binding motif protein 35B
Clonality: Monoclonal
Concentration: 1.0 mg/mL by UV absorbance at 280 nm
Clone Designation: [23A7.C9]
Isotype: IgG1
NCBI: 77411
UniProt: Q8K0G8
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form: Liquid (sterile filtered)
Target: Mouse
Antibody Type: Primary Antibody
Application Dilute: ELISA: 1:750,000, WB: 1:1000
Application Notes: This protein-A purified antibody has been tested for use western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 75.5 kDa in size corresponding to Esrp-1 and 77.4 kDa in size corresponding to
Anti-Esrp-1/2 antibody by western blot shows detection in 293T cell extracts. Lane 1: GFP-transfected. Lane 2: Esrp-1 transfected (arrow). Lane 3: Esrp-2 transfected. Each lane contains approximately 5 µg of lysate. Primary antibody was used at a 1:1000 dilution in PBS-T plus milk, and reacted for 1hr at room temperature. The membrane was washed and reacted with a 1:10,000 dilution of an anti-mouse ECL antibody for 1hr at room temperature. Molecular weight estimation was made by comparison to prestained MW markers.