IL-35 EBI3 Biotin Conjugated Antibody, IgG2b, Clone: [V1.4H6.29], Mouse, Monoclonal

Product Overview

• Article Name: IL-35 EBI3 Biotin Conjugated Antibody, IgG2b, Clone: [V1.4H6.29], Mouse, Monoclonal
• Biozol Catalog Number: ROC-210-306-C55
• Supplier Catalog Number: 210-306-C55
• Alternative Catalog Number: ROC-210-306-C55
• Manufacturer: Rockland Immunochemicals
• Host: Mouse
• Category: Antikörper
• Application: ELISA, IP, WB
• Species Reactivity: Mouse
• Immunogen: Anti-EBI-3 (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with mature full length recombinant mouse EBI-3 produced in E.coli followed by hybridoma development.
• Conjugation: Biotin
• Alternative Names: mouse anti-EBI3 biotin conjugated antibody, mouse anti-IL-35 biotin conjugated antibody, Epstein-Barr virus induced 3 protein, Interleukin-27 subunit beta, IL-27 subunit beta, IL-27B, Epstein-Barr virus-induced gene 3 protein, EBV-induced gene 3 protein, EBI-3, EBI3, IL-35, IL35

Product Properties

• Clonality: Monoclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Clone Designation: [V1.4H6.29]
• Isotype: IgG2b
• NCBI: 50498
• UniProt: O35228
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Mouse
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:10,000, IP: 1µg/mL, WB: 1:1000
• Application Notes: This purified antibody has been tested for use in ELISA, IP, and Western Blot. Specific conditions for reactivity should be optimized by the end user.

Images

Western blot of Anti-Mouse EBI-3 Antibody. Supernatants from cultures containing indicated cells were immunoprecipitated with an anti-p35 monoclonal plus protein G beads, resolved by SDS page gel and blotted with biotinylated anti mouse-EBI-3 clone V1.4H6.29 at 1 µg/mL. The controls were supernatants from 293T cells transfected with either vector only (negative) or vector containing murine IL-35 (positive).

ELISA assay of EBI-3 Antibody. Supernatant from 293T cells transfected with a vector containing murine IL-35 was added to an ELISA plate coated with V1.4H6.29 (210-301-C55) at 2ug/mL in PBS. Mouse EBI-3 was detected with biotinylated V1.4C4.22 (210-301-B66).

Western blot of Biotin conjugated Mouse Anti-EBI-3 (Mouse) antibody. Lane 1: Mouse EBI-3. Lane 2: None. Load: 50 ng per lane. Primary antibody: EBI-3 (Mouse) primary antibody at 1:1,000 for 60 min at RT. Secondary antibody: Peroxidase streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted\Observed size: 25 kDa, 25 kDa for Mouse EBI-3. Other band(s): None.

Immunoprecipitation of Mouse Anti-EBI-3 Antibody. Supernatant from 293T cells transfected with a vector containing murine IL-35 was immunoprecipitated with indicated anti-EBI-3 monoclonal clones V1.4H6.29, V1.4C4.22, and V1.4F5.25 plus Protein G beads, resolved by SDS page gel, and blotted with biotinylated anti-mouse EBI-3 clone V1.4H6.29 at 1µg/mL.