This IgG fraction antibody was prepared from rabbit antiserum after repeated immunizations with recombinant truncated mouse MIP-1alpha protein produced in E.coli.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Lyophilized
Target:
Mouse
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:10,000, WB: 1:1000
Application Notes:
This purified antibody has been tested in western blotting and is suitable in ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 7-8 kDa in size corresponding to the mature 92 amino acid mouse MIP-1
Anti-mouse MIP-1a in western blot shows detection of recombinant mouse MIP-1a/Ccl3 raised in E.coli. Recombinant truncated (0.1 µg, expect ~8kDa) protein was loaded onto and resolved by SDS-PAGE, then transferred to nitrocellulose. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with Rocklands, Inc. Anti-Mouse MIP-1a/Ccl3. After washing, membrane was probed with secondary antibody Dylight(TM) 649 Conjugated Anti-Rabbit IgG (H&L) (Goat) Antibody (611-143-122) diluted 1:20,000 in blocking buffer (p/n MB-070) for 30 min. at RT. Data was collected using Bio-Rad VersaDoc 4000 MP imaging system.
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