Leptin Antibody, Rabbit, Polyclonal

Product Overview

• Article Name: Leptin Antibody, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-210-401-320
• Supplier Catalog Number: 210-401-320
• Alternative Catalog Number: ROC-210-401-320
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human, Mouse
• Immunogen: Anti-Leptin antibody was prepared by repeated immunizations with recombinant mouse leptin 16,000 MW produced in E. coli.
• Conjugation: Unconjugated
• Alternative Names: rabbit anti-leptin antibody, LEP antibody, Leptin Murine Obesity Homolog antibody, Leptin Precursor Obesity Factor antibody, OB antibody, Obese Protein antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• NCBI: 16846
• UniProt: P41160
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Mouse
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:5,000 - 1:20,000, IHC: User Optimized, WB: 1:500 - 1:2,000
• Application Notes: This IgG fraction of anti-Mouse Leptin was tested for use in ELISA and immunoblotting. Reactivity in other immunoassays is unknown. The antibody will recognize recombinant and native 16 kDa leptin from mouse and human.

Images

Western Blot Results of Rabbit Anti-Leptin Antibody. Lane 1: Rec Ms Leptin Reduced [0.03µg].Lane 2: Opal Prestained Molecular Weight Marker (p/n MB-210-0500).Lane 3: Rec Ms Leptin Non-Reduced [0.03µg].Primary Antibody: Rb Anti-Leptin at 1:1000 overnight at 2-8C. Secondary Antibody: Gt Anti-Rabbit IgG [H&L] HRP (p/n 611-1302) at 1:40,000 for 30 mins at RT. Blocking: Universal BlockOut Buffer (p/n MB-073) for 1hr at RT. Predicted MW: ~16kDa. Exposure: 5sec.