IL-27/p28 Antibody Peroxidase Conjugated, HRP, Rabbit, Polyclonal

Product Overview

• Article Name: IL-27/p28 Antibody Peroxidase Conjugated, HRP, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-210-403-B54
• Supplier Catalog Number: 210-403-B54
• Alternative Catalog Number: ROC-210-403-B54
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: WB
• Species Reactivity: Mouse
• Immunogen: This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant mouse IL27/p28 protein.
• Conjugation: HRP
• Alternative Names: rabbit anti-IL-27/p28 peroxidase conjugated antibody, rabbit anti-interleukin 27a peroxidase conjugated antibody, Interleukin-27 subunit alpha, IL-27 subunit alpha, IL-27-A cytokine, IL27-A, p28, IL-30

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• NCBI: 246779
• UniProt: Q8K3I6
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Mouse
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:5,000
• Application Notes: IL-27 is expressed in activated antigen presenting cells including monocytes, endothelial cells, and dendritic cells, for example mouse CD4 splenocytes. This purified antibody has been tested for use in western blotting. Specific conditions for reactivit

Images

Recombinant mouse IL27/p28 was loaded at 0.25 µg. The blot was blocked with 1% BSA in TBST for 30 min at RT. Blot was incubated with HRP rabbit anti-Mouse IL-27/p28 in 1% BSA/TBST at 1:5,000 for 30 min at RT. Data was collected using Bio-Rad VersaDoc 4000 MP.