IL-1 Beta Antibody Biotin Conjugated, Rabbit, Polyclonal

Product Overview

• Article Name: IL-1 Beta Antibody Biotin Conjugated, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-210-406-319
• Supplier Catalog Number: 210-406-319
• Alternative Catalog Number: ROC-210-406-319
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: DOT, WB
• Species Reactivity: Mouse
• Immunogen: This antibody was prepared by repeated immunizations with recombinant mouse IL-1ß produced in E.coli. The MW of recombinant mouse IL-1ß was 17 kDa.
• Conjugation: Biotin
• Alternative Names: rabbit anti-IL-1 beta biotin conjugated antibody, rabbit anti-IL-1b biotin conjugated antibody, rabbit anti-Interleukin-1 beta antibody, IL-1ß, catabolin

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• NCBI: 16176
• UniProt: P10749
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Mouse
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:20,000-1:100,000, Flow Cytometry: User Optimized, IHC: 1:1,000-1:5,000, IP: User Optimized, WB: 1:2,000-1:10,000
• Application Notes: Anti-Mouse IL-1ß has been tested in dot blot and western blot and is suitable for use in neutralizations, ELISA, radioimmunoassays, flow cytometry, immunohistochemistry, and immunoprecipitation. It recognizes the 17,000 MW mature IL-1ß. For immunoblots,

Images

Western Blot of Rabbit anti-Mouse IL-1ß Antibody Biotin Conjugated. Lane 1: Mouse IL-1ß Recombinant Protein. Load: 50 ng per lane. Primary antibody: Rabbit anti-Mouse IL-1ß Antibody Biotin Conjugated at 1:1,000 overnight at 4C. Secondary antibody: HRP streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 minutes at RT. Predicted/Observed size: 17.5 kDa, 17.5 kDa for proteolytically processed Mouse Il-1ß.