IL-27 is expressed in activated antigen presenting cells including monocytes, endothelial cells, and dendritic cells, for example mouse CD4 splenocytes. This purified antibody has been tested for use in western blotting. Specific conditions for reactivit
Western Blot of Biotin Conjugated Rabbit anti-IL-27/p28 antibody. Lane 1: Mouse IL-27/p28. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Biotin rabbit secondary antibody at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 24 kDa, 24 kDa for Mouse IL-27/p28. Other band(s): None.
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