Anti-MIP-3alpha (RAT) Monoclonal Antibody was produced in rat by repeated immunizations with mature full length recombinant mouse MIP-3alpha produced in E.coli followed by hybridoma development.
Conjugation:
Biotin
Alternative Names:
rat anti-MIP-3alpha biotin conjugated antibody, CCL20, C-C motif chemokine 20, small-inducible cytokine A20, macrophage inflammatory protein 3 alpha, MIP-3-alpha, liver and activation-regulated chemokine, CC chemokine LARC and beta chemokine exodus-1
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Lyophilized
Target:
Mouse
Antibody Type:
Primary Antibody
Application Dilute:
WB: 0.5µg/mL
Application Notes:
This purified antibody has been tested for use in ELISA, IP, western blot, and Neutralization. Specific conditions for reactivity should be optimized by the end user.
Western Blot of Mouse MIP 3alpha (RAT) antibody Lane 1: mouse recombinant MIP-3a Lane 2: human recombinant MIP-3a Primary antibody: MIP 3alpha antibody at 0.1 ug/ml for overnight at 4C Secondary antibody: IRDye800(TM) goat anti-rat at 1:10,000 for 45 min at RT Block: 5% BLOTTO overnight at 4C
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