IL-35 EBI3 Biotin Conjugated Antibody, Clone: [10J811], Rat, Monoclonal

Product Overview

• Article Name: IL-35 EBI3 Biotin Conjugated Antibody, Clone: [10J811], Rat, Monoclonal
• Biozol Catalog Number: ROC-210-506-B66
• Supplier Catalog Number: 210-506-B66
• Alternative Catalog Number: ROC-210-506-B66
• Manufacturer: Rockland Immunochemicals
• Host: Rat
• Category: Antikörper
• Application: FC
• Species Reactivity: Mouse
• Immunogen: Anti-EBI-3 (RAT) Monoclonal Antibody was produced in rat by repeated immunizations with mature full length recombinant mouse EBI-3 produced in E.coli followed by hybridoma development.
• Conjugation: Biotin
• Alternative Names: rat anti-IL-35 biotin conjugated antibody, rat anti-EBI3 biotin conjugated Antibody, Interleukin-27 subunit beta, IL-27 subunit beta, IL-27B, Epstein-Barr virus-induced gene 3 protein, EBV-induced gene 3 protein, EBI3, EBI-3, IL-35, IL35

Product Properties

• Clonality: Monoclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Clone Designation: [10J811]
• NCBI: 50498
• UniProt: O35228
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Mouse
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: User Optimized, Flow Cytometry: 1.5-3.0µg/10x6 cells, WB: 1µg/mL
• Application Notes: This purified antibody has been tested for use in ELISA, Western Blot, and Flow Cytometry. Specific conditions for reactivity should be optimized by the end user.

Images

Western Blot of Mouse Anti-EBI-3 Monoclonal Antibody. A) 0.1 ug/mL on recombinant protein. B) 3 ug/ml on recombinant mouse IL-35 protein. Goat anti-rat IgG HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Flow Cytometry of Anti-Mouse EBI-3 Antibody Figure A: Intracellular flow cytometry of mouse EBI3 in transfected and untransfected HEK 293 cells (Brefeldin A treated, 5 hours) using mouse EBI3 antibody at 3 ug/10 6 cells. Figure B: Intracellular flow cytometry of mouse EBI3 in stimulated and unstimulated RAW cells (LPS treated, 50 ng/mL, overnight) using mouse EBI3 antibody at 1.5 ug/10 6 cells.