Rat Serum Antibody Peroxidase Conjugated, HRP, Rabbit, Polyclonal

Catalog Number: ROC-212-4301S
Article Name: Rat Serum Antibody Peroxidase Conjugated, HRP, Rabbit, Polyclonal
Biozol Catalog Number: ROC-212-4301S
Supplier Catalog Number: 212-4301S
Alternative Catalog Number: ROC-212-4301S
Manufacturer: Rockland Immunochemicals
Host: Rabbit
Category: Antikörper
Application: DOT, ELISA
Species Reactivity: Rat
Immunogen: Rat Serum Proteins
Conjugation: HRP
Alternative Names: rabbit anti-Rat Serum Antibody Peroxidase Conjugated, rabbit anti-Rat Serum HRP conjugated Antibody
Clonality: Polyclonal
Concentration: 1.0 mg/mL by UV absorbance at 280 nm
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form: Liquid (sterile filtered)
Target: Rat
Antibody Type: Primary Antibody
Application Dilute: ELISA: User Optimized, IHC: User Optimized, WB: User Optimized
Application Notes: Anti-Rat serum Peroxidase Conjugated antibody has been tested by Dot blot and ELISA and is suitable for western blotting, IP and for ELISA. Researchers should determine optimal titers for applications that are not stated below.
ELISA Results of Rabbit Anti-Rat Serum Antibody Peroxidase Conjugated. Each well was coated in duplicate with 1.0 µg of Rat IgG (p/n 012-0102) [Red Line]. The working dilution is 20,000. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using TMB substrate (p/n TMBE-1000).