Anti-NF-M Monoclonal Antibody was produced by repeated immunizations with recombinant C-terminus part of NF-M expressed in E. coli.
Conjugation:
Unconjugated
Alternative Names:
chicken anti-Neurofilament M antibody, Neurofilament medium polypeptide, NF-M, 160 kDa neurofilament protein, Neurofilament 3, Neurofilament triplet M protein
Clonality:
Polyclonal
Concentration:
Titrated value sufficient to run approximately 10 mini blots.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid
Target:
Rat
Antibody Type:
Primary Antibody
Application Dilute:
IF Microscopy: 1:1000, WB: 1:5000
Application Notes:
Anti- Neurofilament M antibody is tested for use in Western Blotting and IF. Specific . Expect a band of approximately 145 kDa in size corresponding to NF-M proteins in the appropriate cell lysate or extract. Researchers should determine optimal titers f
Western Blot of Chicken anti-Neurofilament M antibody. Lane 1: Rat cortex lysate. Lane 2: none. Load: 20 µg per lane. Primary antibody: Neurofilament M antibody at 1:5,000 for overnight at 4C. Secondary antibody: IRDye800(TM) chicken secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 145 kDa for Neurofilament M. Other band(s): none.
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